PHYSICAL TEST AND LETHAL CONCENTRATION 95 (LC95) ASSAY OF KAFFIR LIME SKIN GRANULE EXTRACT (Citrus hystrix DC.) ON Aedes aegypti MOSQUITO LARVAE AS A NEW CANDIDATE BIOINSECTICIDE

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DWI WAHYUNI
https://orcid.org/0000-0003-4742-3599
ADITYA KURNIAWAN
LIDYA YUSTIKA PUTRI

Abstract

Indonesia needs to be encouraged by the discovery of new bioinsecticides substituting synthetic insecticides, which have posed a danger to human survival. One of the plants that have the potential to become bioinsecticides is kaffir lime (Citrus hystrix DC). On the skin’s kaffir lime contains chemical compounds such as secondary metabolites and essential oils. The most considerable and largest content of kaffir lime essential oil is limonene or limonoid. This compound is a member of monoterpenes which has vigorous insecticide activity. Limonene/limonoid act as stomach poisons of mosquito larvae. Limonene/limonoid enters the body of the larvae through immersion of the consumed extract concentration. This research aims to determine the right formulation and specifications through physical properties test and determine the lethal concentration 95 ( ) of kaffir lime granule extract (Citrus hystrix DC) against Aedes aegypti mosquito larvae so that it meets the requirements as a new bioinsecticide. Physical tests carried out include tests of water content, flow time test, moisture content test, indexing, and fragility test. Based on the results of formulations and specifications through physical properties test, the water content of granules is 0.80-1.40%, the flow time is 7.00, the moisture content is 3.40, the index is 7.11, and the fragility is 0.2. While the results of probit analysis obtained the value of  of kaffir lime skin extracts (Citrus hystrix DC.) for 24 hours of bran is 7915.85 ppm with a lower limit of 6802.33 ppm and an upper limit of 9718.21 ppm.

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How to Cite
WAHYUNI, D., KURNIAWAN, A., & PUTRI, L. Y. (2021). PHYSICAL TEST AND LETHAL CONCENTRATION 95 (LC95) ASSAY OF KAFFIR LIME SKIN GRANULE EXTRACT (Citrus hystrix DC.) ON Aedes aegypti MOSQUITO LARVAE AS A NEW CANDIDATE BIOINSECTICIDE. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(31-32), 57-64. Retrieved from https://www.ikppress.org/index.php/PCBMB/article/view/6243
Section
Original Research Article

References

Wikandari RJ, Surati S. The effect of kaffir lime (Citrus Hystrix) essential oil on behavior and Mortality of Aedes Aegypti Larvae, J. Ris. Kesehat. 2020;9(1):6–11.

Sanyaolu A, et al. Global epidemiology of dengue hemorrhagic fever: An Update, J. Hum. Virol. Retrovirology. 2017;5:6.

Gnankiné O, Bassolé I. Essential oils as an alternative to pyrethroids’ resistance against anopheles species complex giles (Diptera: Culicidae). Molecules. 2017;22:1321.

Satriawan D, Sindjaja W, Richardo T. Toxicity of the organophosphorus pesticide temephos, Indones. J. Life Sci. 2019;01(02).

Mya MM, Aung ZZ, Win KKL, New CT, et al. Larvicidal properties of citrus hystrix dc fruit (taw-shaut-khar-thee) extracts against aedes aegypti larvae collected from different townships of yangon. Myanmar Heal. Sci. Res. J. 2019;31:23–29.

Sihombing JR, Dharma A, Chaidir Z, Fachrial E, Munaf E. Phytochemical screening and antioxidant activities of 31 fruit peel extract from Sumatera, Indonesia. 2015;7:190–196.

Srifuengfung S, Bunyapraphatsara N, Satitpatipan V, Tribuddharat C, Junyaprasert VB, Tungrugsasut W, et al. Antibacterial oral sprays from Kaffir lime (Citrus hystrix DC.) fruit peel oil and leaf oil and their activities against respiratory tract pathogens. J. Tradit. Complement. Med; 2019.

Mya MM, Aye YY, AW Oo, Saxena RK. Effect of citrus hystrix DC leaves ethanol extract on larvae of aedes aegypti, J. Biol. Eng. Res. Rev. 2015;2(2)1–6.

Da Botas GS, Cruz RAS, De Almeida FB, Duarte JL, Araújo RS, Souto RNP, et al. Limonene Nanoemulsions: Promising larvicidal agents for aedes aegypti (Diptera: Culicidae) Control. Molecules. 2017;22:1–14.

Wahyuni D, Susanti DA, Elmaghfuroh DR. The physical properties and phytochemical exploration of bioinsecticide granules mixed betel leaf extract (Piper betle) and srikaya seed extract (Annona squamosa). Int. J. Sci. Technol. Res. 2019;8:242–247.

Wahyuni D, Sulistyaningsih E, Yaswinda DQ, Waluyo J. Toxicity effect of granular bioinsecticide mixture of betel leaf (Piper betel) and srikaya seed (Annona squamosa). IOP Conf. Ser. Earth Environ. Sci. 2019;243:012096.

Wahyuni D. new bioinsecticide granules toxin from ectract of papaya (Carica papaya) Seed and leaf modified against aedes aegypti larvae. Procedia Environ. Sci. 2015;23:323–328.

Abdullah SSS, Othman MKI. Sustainable technologies for the management of agricultural wastes, Sustain. Technol. Manag. Agric. Wastes; 2018.

Jutkus RAL, Li N, Taylor LS , Mauer LJ. Effect of temperature and initial moisture content on the chemical stability and color change of various forms of vitamin C. Int. J. Food Prop. 2015;18:862–879.

Wijaya YA, Widyadinata D, Irawaty W, Ayucitra A. Fractionation of Phenolic Compounds from Kaffir Lime (Citrus Hystrix) Peel Extract and Evaluation of Antioxidant Activity, Reaktor. 2017;17(3):111.

Shanmugam S. Granulation techniques and technologies: Recent progresses. Bio Impacts. 2015;5:55–63.

Crouter A, Briens LL. The effect of moisture on the flowability of pharmaceutical excipients. AAPS Pharm Sci Tech. 2014;15:65–74.

Yahya A, Amadi I, Hashib SA, Mustapha FA. Influence sample sizing of citrus hystrix essential oil from hydrodistillation extraction, IOP Conf. Ser. Mater. Sci. Eng. 2018;334(1)4–10.

Srifuengfung S, et al. Antibacterial oral sprays from kaffir lime (Citrus hystrix DC.) fruit peel oil and leaf oil and their activities against respiratory tract pathogens, J. Tradit. Complement. Med; 2019.

Tanzil L, Latirah, Nugroho D. Anti dandruff activity of extracts from kaffir lime (Citrus hystrix dc.) Prepared by Different Solvents. 2017;8843:57–62.

Hartesi B, Sriwidodo, Abdassah M, Chaerunisaa AY. Starch as pharmaceutical excipient. Int. J. Pharm. Sci. Rev. Res. 2016;41:59–64.

Li J, Y Wu Y. Lubricants in pharmaceutical solid dosage forms. Lubricants. 2014;2:21–43.

Nur A, Ansori M, Supriyadi AP, Kartjito MV, Adrianto H. Biolarvicidal effectivities of polar and non-polar extract fraction from kaffir lime (Citrus hystrix) Leaf against 3. J. Biol. Eng. Res. Rev. 2015;2:14–17.