SALT TOLERANT CELLS SELECTION OF POTATO CALLUS FROM CULTURING AT DIFFERENT CONCENTRATIONS OF SODIUM CHLORIDE BY in vitro CULTURE TECHNIQUE
PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY,
The study was carried out in the laboratory of Plant Tissue Culture at the Faculty of Agriculture, University of Basra. Tubers of three certified Dutch cultivars of potato plant (Lizita, Arnova and Safari) were brought from the Horticulture Station for Potato Seed Production Project, Babel city, Iraq. The aim was to produce salinity- tolerant cells from the callus of three cultivars of potato Lizita, Arnova and Safari cultured on MS medium containing different concentrations of sodium chloride. The results of the study showed that callus of Lizita cultivar grew when cultured on MS media supplemented with 0, 80 and 100 mmol.L-1 NaCl and did not grow at 120, 140 and 160 mmol.L-1 NaCl after four weeks of culture. The Lizita and Arnova cultivars gave the highest fresh weight of callus compared with Safari cultivar after four weeks of culture. The control and 0.250 mmol.L-1 salicylic acid + 120 mmol.L-1 NaCl treatments improved the callus growth of Lizita cultivar after six weeks from culturing. However, when the callus of Lizita cultivar was subjected to NaCl at a concentration of 80 mmol.L-1, only one additional protein band appeared on the gel. This treatment also indicated the appearance of four proteins with high molecular weight in the callus of Lizita cultivar, reached 225.000, 150.000, 100.000 and 75.000 kDa.
- Abiotic stress
- dry weight
- protein pattern
- salicylic acid.
How to Cite
Chen Q, Nandy SJ, Kereliuk G. Screening potato genotypes for antioxidant capacity and total phenolics. Annual meeting of CPS-SCP (with plant Canada, 2007). Saskatoon, SK, Canada. 2007;142:10-14.
FAO. Food and Agriculture Organization; 2013.
Al-Zubaidi AH. The salinity of soil. Theoretical and applied basics. ministry of higher education and scientific research, Bait Al-Hakamah Press, University of Baghdad, Iraq (In Arabic); 1989.
Mass EV, Hoffman GJ. Crop salt tolerance current assessment. J. Irrig. Drain. Div. 1977;103(2):115-134.
Shah MI, Jabeen M, Ilahi I. In vitro callus induction, its proliferation and regeneration in seed explants of wheat (Triticum aestivum L.). Pak. J. Bot. 2003;35(2):209-217.
Munns R. Genes and salt tolerance: Bringing them together. New Phytol. 2005; 167:645-663.
Hayat Q, Hayat S, Irfan M, Ahmad A. Effect of exogenous salicylic acid under changing environment: A review. Exp. Bot. 2010;68:14- 25.
Kim MJ, Lim GH, Kim ES, Ko CB, Yang KY, Jeong JA, Lee MC, Kim CS. Abiotic and biotic stress tolerance in Arabidopsis overexpressing the multiprotein bridging factor 1a (MBF1A) transcriptional co-activator gene. Biochem. Biophys. Res. Common. 2007;354(2):440-446.
Sajid ZM, Aftab F. Role of salicylic acid in amelioration of salt tolerance in potato (Solanum tuberosum L.) under In vitro conditions. Pak. J. Bot. 2012;44:37- 42.
Queiros F, Fidalgo F, Santos I, Salema R. In vitro selection of salt tolerant cell lines in Solanum tuberosum L. Biol. Plant. 2007; 51:728-734.
Mohamed AA, Matter MA, Saker M. Effect of salt stress on some defense mechanisms of transgenic and wild potato clones (Solanum tuberosum L.) grown in vitro. Nat. and Sci. 2010;8(12):181-193.
Forooghian S, Esfarayeni S. An evaluation of the effect of salt stress on callus induction in different potato cultivars. American-Eurasian J. Agric. & Environ. Sci. 2013;13(8):1135-1140.
Hassanein AM. Effect of relatively high concentration of mannitol and sodium chloride on regeneration and gene expression of stress tolerant (Alhagi graecorum) and stress sensitive (Lycopersicon esculentum) Plant species. Bulg. J. Plant Physiol. 2004;30(3-4):19-36.
Yamaguehi T, Blumwad E. Developing salt tolerance crop plants: Challenges and opportunities. Plant Sci. 2005;10(12):615-620.
Amini F, Ehsanpour AA, Hoang OT, Shin J. Protein pattern changes in tomato under In vitro salt stress. Russian J. Plant Physiol. 2007;54(4):464-471.
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant.1962; 15:473 497.
Bavi V, Shiran B, Khodambashi M, Ranjbar A. Protein electrophoresis profiles and physiochemical indicators of salinity tolerance in sorghum (Sorghum bicolor L.). Afr. J. Biotechnol. 2011;10(14):2683-2697.
Snedecor GM, Cochran WG. Statistical Methods. 9th ed., The Iowa State University, American Press, Iowa, U.S.A. 1986;507.
Munns, R. Comparative physiology of salt and water stress. Plant Cell and Environ. 2002;25:239-250.
Orcutt DM, Nilsen ET. The physiology of plant under stress: Soil and biotic factors. John Wiley & Sons, Inc., USA; 2000
Sakhabutdinova AR, Fatkhutdinova PR, Bezrukova MV, Shakirova FM. Salicylic acid prevents the damaging action of stress factors on wheat plants. Bulg. J. Plant Physiol. 2003;269: 314-319.
Shakirova FM, Sakhabutdinova AR, Bezrukova MV, Fatkhutdinova RA, Fatkhudinova PR. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Sci. 2003; 164(3):317-322.
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