PRODUCTION OF LIGNOCELLULOLYTIC ENZYMES BY Aspergillus niger FROM BROWN MID RIB Sorghum VARIETIES
PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY,
Page 47-56
Abstract
The present study reported with the production of enzyme cellulases (CMCase, Fpase and β-glucosidase) and xylanase by submerged fermentation using Aspergillus niger and sorghum hybrid non-BMR CSH 22 variety & low lignin BMR 22 and 24 substrates were evaluated after pre-treating with H2SO4 & NaOH. The highest cellulose and low lignin reported pretreatment conditions were applied for substrates and used the same in the fermentation. Maximum CMCase, FPase, and β-glucosidase production was observed for pretreated BMR 22 Sorghum biomass with values 30.5 ± 3.75 IU/ml, 2.74±0.34 IU/ml, and 9.6 ± 0.37 IU/ml respectively. But maximum xylanase production 15.8±1.75 IU/ml was observed while using untreated CSH 22 sorghum biomass as substrate. Results indicate low lignin mutant BMR22 and 24 substrates were promising substrates for the production of cellulase enzymes.
Keywords:
- Sorghum
- Brown Mid Rib
- cellulose
- xylanase
- submerged fermentation
How to Cite
KUMAR, P. S., GANDHAM, V., UMA, A., & UMAKANTH, A. V. (2020). PRODUCTION OF LIGNOCELLULOLYTIC ENZYMES BY Aspergillus niger FROM BROWN MID RIB Sorghum VARIETIES. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(37-38), 47-56. Retrieved from https://www.ikppress.org/index.php/PCBMB/article/view/5410
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Bakari Y, Jacques P, Thonart P, Xylanase production by Penicillium canescens 10-10c in solid-state fermentation, Appl Biochem Biotechnol. 2003;105:737-747.
Suprabha GN, Sindhu R, Shashidhar S. Fungal xylanase production under solid state and submerged fermentation conditions. African Journal of Microbiology Research. 2008;2:082-086.
Costa-Ferreira M, Diass A, Maximo C, Morgado MJ, Sena-Martins G, Duarte JC, Xylanolytic enzyme production by an A. niger isolate. Appl. Biochem. Biotechnol. 1994;44:231-242.
Bailey MJ, Viikari L, Production of xylanase by Aspergillus fumigatus and Aspergillus oryzae on xylan-based media. W. J. Microbiol. Biotechnol. 1993;9:80-8.
Sonia C, Saini HS, Sorghum straw for xylanase hyper-production by Thermomyces lanuginosus (D2W3) under solid-state fermentation, Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, India; 2005.
Modi Acharya A, Chabhadiya Shah PB, Amitkumar Acharya DK, Process optimization for xylanase production by A. niger in solid state fermentation, International Journal of Biotechnology and Biosciences. 2011;1:423-430.
Salihu A, Abbas O, Sallau AB, Alam MZl. Agricultural residues for cellulolytic enzyme production by Aspergillus niger: Effects of pretreatment, Biotech. 2015;5: 1101-1106.
Oliveira rodrigues, Patrisia Santos, Beatriz Costa, Leticia Alves Henrique, Mariana Pasquini, Daniel Alves Baffi, Milla. Xylanase and β-glucosidase production by Aspergillus fumigatus using commercial and lignocellulosic substrates submitted to chemical pre-treatments, Industrial Crops and Products. 2016;95.
Mrudula S, Murugammal R, Production of cellulase by A. niger under submerged and solid state fermentation using coir waste as a substrate. Brazilian Journal of Microbiology. 2011:42:1119-27.
Gaurav VS, Rina DK, Kishore SR, Thermostable xylanase production and partial purification by solid-state fermentation using agricultural waste wheat straw, Mycology. 2010;1(2):106-112.
Poppy EM, Leonardo DG, Simon JM, Unlocking the potential of lignocellulosic biomass through plant science, New Phytologist. 2016;209:1366-1381.
Demirbaş A, Biofuels sources, biofuel policy, biofuel economy and global biofuel projections, energy convers. Manag. 2008; 49:2106-2116.
Belum Reddy VS, Srinivasa Rao P, Ashok Kumar A, Sanjana Reddy P, Parthasarathy Rao P, Kiran Sharma K, Michael Blummel, Ch. Ravinder Reddy, Sweet sorghum as a biofuel crop: Where are we now? ICRISAT; 2009.
Rao SS, Seetharama N, Ratnavathi Umakanth CV, Monika Dalal AV. Second generation biofuel production from sorghum biomass. Directorate of Sorghum Research, ICAR, Rajendranagar, Hyderabad (AP) India; 2010.
Corredor DY, Salazar JM, Hohn KL, Bean S, Bean BW, Wang D, Evaluation and characterization of forage sorghum as feedstock for fermentable sugar production, App. Biochem. Biotechnol. 2009;158:164-79.
Sattler SE, FunnellHarris DL, Pedersen JF, Efficacy of singular and stacked brown midrib 6 and 12 in the modification of lignocellulose and grain chemistry, J Agric Food Chem. 2010;58:3611–6.
Schmer MR, Vogel KP, Varvel GE, Follett RF, Mitchell RB, Jin VL, Energy potential and greenhouse gas emissions from bioenergy cropping systems on marginally productive cropland, PLoS One. 2014;9(3): 1-8.
Noura El-Ahmady El-Naggar, Sahar Deraz and Ashraf Khalil, Bioethanol production from lignocellulosic feedstocks based on enzymatic hydrolysis: Current status and recent developments, biotechnology, 2014; 13:1-21.
Manan MA, Webb C. Design aspects of solid state fermentation as applied to microbial bioprocessing. J Appl Biotechnol Bioeng. 2017;4(1):511-532.
Ande P, Mishra C, Farrell R, Eriksson KE, Redox interactions in lignin degradation: Interaction between laccase, different peroxidases and cellobiose: Quinine oxidoreductase. J. Biotechnol. 1990;13: 189-198.
Gilbert HJ, Hazlewood GP, Bacterial cellulases and xylanases, J. Gen. Microbiol. 1993;139:187-194.
Maheswari U, Chandra TS, Production and potential applications of a xylanase from a new strain of Streptomyces cuspidosporus, World J Microb Biot. 2000;16:257-263.
Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M. Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour. Technol. 2005;96:673-686.
Iroba KL, Tabil LG, Sokhansanj S, Meda V, Producing durable pellets from barley straw subjected to radio frequency-alkaline and steam explosion pretreatments, Int J AgrBiolEng. 2014;7(3):68-82.
Praveen Kumar Reddy G, Narasimha G, Dileep Kumar K, Ramanjaneyulu G. Cellulase production by A. niger on different natural lignocellulosic substrates, Int. J. Curr. Microbiol. App. Sci. 2015;4:835-845.
Polizeli ML, Rizzatti ACSR, Monti Terenzi HF, Jorge JA, Amorim DS. Xylanases from fungi: Properties and industrial applications. Applied Microbiology and Biotechnology. 2005;67(5):577–591.
Aliyah A, Alamsyah G, Ramadhani R, Hermansyah H, Production of α-Amylase and β-Glucosidase from A. niger by solid state fermentation method on biomass waste substrates from rice husk, bagasse and corn cob. Energy Procedia. 2017;136: 418-423.
Anonymous, technical association of the pulp and paper industry: Sampling and preparing wood for analysis technical association of the pulp and paper industry. TAPPI Standard T203 cm-99, Atlanta, USA; 1999.
Palakeerti Srinivas Kumar, Palety Kiran Kumar, Addepally Uma, Umakanth AV. (In press) Res. J. Biotech.
M, Mandel RA, Roche C, Measurement of saccharifying cellulase. Biotechnol Bioeng Symp. 1976;6:21-23.
Ghose TK. Measurement of cellulase activities. Pure & Appl. Chem. 1987;59: 257-268.
Microsoft Press Div. of Microsoft Corp. One Microsoft Way Redmond, WA United States; 2020.
Kshirsagar SD, Bhalkar BN, Waghmare PR, Saratale GD, Saratale RG, Govindwar SP, Sorghum husk biomass as a potential substrate for production of cellulolytic and xylanolytic enzymes by Nocardiopsis sp. KNU. Biotech. 2017;7(3):163.
Asmaa Abdella, Tarek El-Sayed, Mazeed Ashraf F, El-Baz Shang-Tian Yang. Production of -glucosidase from wheat bran and glycerol by A. niger in stirred tank and rotating fibrous bed bioreactors, Process Biochemistry. 2016;51(10):1331-1337.
Cuiyi Liang, Xu Huijuan, Zhang Yu, Guo Ying, Chen Xiaoyan, Yuan Zhen-Hong, He Min-Chao, Xu Jing-Liang. Induction of highly active beta-glucosidase from biomass material and its application on lignocellulose hydrolysis and fermentation. Cellulose Chemistry and Technology. 2017;51:719-729.
Bakari Y, Jacques P, Thonart P, Xylanase production by Penicillium canescens 10-10c in solid-state fermentation, Appl Biochem Biotechnol. 2003;105:737-747.
Suprabha GN, Sindhu R, Shashidhar S. Fungal xylanase production under solid state and submerged fermentation conditions. African Journal of Microbiology Research. 2008;2:082-086.
Costa-Ferreira M, Diass A, Maximo C, Morgado MJ, Sena-Martins G, Duarte JC, Xylanolytic enzyme production by an A. niger isolate. Appl. Biochem. Biotechnol. 1994;44:231-242.
Bailey MJ, Viikari L, Production of xylanase by Aspergillus fumigatus and Aspergillus oryzae on xylan-based media. W. J. Microbiol. Biotechnol. 1993;9:80-8.
Sonia C, Saini HS, Sorghum straw for xylanase hyper-production by Thermomyces lanuginosus (D2W3) under solid-state fermentation, Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, India; 2005.
Modi Acharya A, Chabhadiya Shah PB, Amitkumar Acharya DK, Process optimization for xylanase production by A. niger in solid state fermentation, International Journal of Biotechnology and Biosciences. 2011;1:423-430.
Salihu A, Abbas O, Sallau AB, Alam MZl. Agricultural residues for cellulolytic enzyme production by Aspergillus niger: Effects of pretreatment, Biotech. 2015;5: 1101-1106.
Oliveira rodrigues, Patrisia Santos, Beatriz Costa, Leticia Alves Henrique, Mariana Pasquini, Daniel Alves Baffi, Milla. Xylanase and β-glucosidase production by Aspergillus fumigatus using commercial and lignocellulosic substrates submitted to chemical pre-treatments, Industrial Crops and Products. 2016;95.
Mrudula S, Murugammal R, Production of cellulase by A. niger under submerged and solid state fermentation using coir waste as a substrate. Brazilian Journal of Microbiology. 2011:42:1119-27.
Gaurav VS, Rina DK, Kishore SR, Thermostable xylanase production and partial purification by solid-state fermentation using agricultural waste wheat straw, Mycology. 2010;1(2):106-112.
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