PRODUCTION OF LIGNOCELLULOLYTIC ENZYMES BY Aspergillus niger FROM BROWN MID RIB Sorghum VARIETIES
PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY,
The present study reported with the production of enzyme cellulases (CMCase, Fpase and β-glucosidase) and xylanase by submerged fermentation using Aspergillus niger and sorghum hybrid non-BMR CSH 22 variety & low lignin BMR 22 and 24 substrates were evaluated after pre-treating with H2SO4 & NaOH. The highest cellulose and low lignin reported pretreatment conditions were applied for substrates and used the same in the fermentation. Maximum CMCase, FPase, and β-glucosidase production was observed for pretreated BMR 22 Sorghum biomass with values 30.5 ± 3.75 IU/ml, 2.74±0.34 IU/ml, and 9.6 ± 0.37 IU/ml respectively. But maximum xylanase production 15.8±1.75 IU/ml was observed while using untreated CSH 22 sorghum biomass as substrate. Results indicate low lignin mutant BMR22 and 24 substrates were promising substrates for the production of cellulase enzymes.
- Brown Mid Rib
- submerged fermentation
How to Cite
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